Microtome Operation Procedure

Release time:

2025-01-10 10:20

I. Pre-preparation

1. Ensure that the working environment is clean and dry to avoid the generation of dust in the process of slicing to affect the quality of slicing.
2. Prepare the necessary tools and materials, including microtome, blades, fixative, ice table, samples, etc.

Power on the machine and check

1. Turn on the power supply of the microtome and check whether the equipment is running normally. 2.
2. Check whether the blades are sharp and need to be replaced. 3.
3. Adjust the thickness of the slices as needed to ensure uniform slicing.

Sample Handling

1. Fix the samples to be cut on the ice table to ensure that the samples are stable. 2.
2. Adjust the concentration of fixative according to the characteristics of the sample to ensure that the sample will not be deformed or shifted during the slicing process.

Start slicing

1. Place the fixed sample on the carrier table of the slicer.
2. Adjust the position of the slicer so that the blade is in contact with the sample.
3. Start slicing, pay attention to the speed and strength to avoid vibration or damage to the blade.
4. During the slicing process, observe the quality of the slices and make adjustments if necessary.

V. Post-processing

1. After slicing, place the slices in a suitable container. 2.
2. Clean the slicer and keep the equipment clean. 3.
3. further process the slices, such as staining and sealing.

Precautions

1. Pay attention to safety during operation to avoid blade cuts.
2. The parameter settings of the slicer should be adjusted according to the characteristics of the samples to ensure the quality of the slices.
3. Check the performance of the equipment regularly to ensure that the equipment is normal.

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